- Genes & Diseases,Volume 2, Issue 3,2014, Pages 276-281
- Correlation between KCNQ1 and KCNJ11 gene polymorphisms and type 2 and post-transplant diabetes mellitus in the Asian Indian population
- Imran Ali Khan1,2,3, Kiran Kumar Vattam1, Parveen Jahan3, Kamal Kiran Mukkavali4, Qurratulain Hasan1,2, Pragna Rao5
- 1.Department of Genetics and Molecular Medicine, Kamineni Hospitals, Hyderabad, India;2.Department of Genetics, Vasavi Medical and Research Centre, Khairathabad, Hyderabad, India;3.Department of Genetics and Biotechnology, Osmania University, Tarnaka, Hyderabad, India;4.Department of Nephrology, Kamineni Hospitals, Hyderabad, India;5.Department of Biochemistry, Kasturba Medical College, Manipal University, Manipal, Karnataka, India
Type 2 diabetes mellitus (T2DM) and post-transplant diabetes mellitus (PTDM) share a common pathophysiology. However, diabetes mellitus is a complex disease, and T2DM and PTDM have different etiologies. T2DM is a metabolic disorder, characterized by persistent hyperglycemia, whereas PTDM is a condition of abnormal glucose tolerance, with variable onset after organ transplant. The KCNQ1 and KCNJ11 gene encode potassium channels, which mediate insulin secretion from pancreatic β-cells, and KCN gene mutations are correlated with the development of diabetes. However, no studies have been carried out to establish an association between KCNQ1 and KCNJ11 gene polymorphisms and T2DM and PTDM. Therefore, our study was aimed at the identification of the role of KCNQ1 and KCNJ11 gene polymorphisms associated with T2DM and the risk of developing PTDM in the Asian Indian population. We have carried out a case–control study including 250 patients with T2DM, 250 control subjects, 42 patients with PTDM and 98 subjects with non-PTDM. PCR-RFLP analysis was carried out following the isolation of genomic DNA from EDTA-blood samples. The results of the present study reveal that two single nucleotide polymorphisms (rs2283228 and rs5210, of the KCNQ1 and KCNJ11 genes, respectively) are associated with both T2DM and PTDM. The results of our study suggest a role of KCNQ1 and KCNJ11 gene variants in the increased risk of T2DM and PTDM in the Asian Indian population.
Asian Indians; KCNJ11; KCNQ1; PTDM; T2DM
Copyright © 2014 Chongqing Medical University. Published by Elsevier B.V